3,3′,5,5′-Tetramethylbenzidine

Product Description

•     Cas Number 54827-17-7
•     Linear Formula [-C6H2(Ch3)2-4-Nh2]2
•     Molecular Weight 240.34

• Grade   :    Analytical Standard
•       Standard For Gc
• Assay    :   ≥99.0% (Gc)
• Shelf Life  :     Limited Shelf Life, Expiry Date On The Label
• Mp     :  166-171 °C
•       168-171 °C(Lit.)

Description

Application

3,3′,5,5′-Tetramethylbenzidine (Tmb) Is A Noncarcinogenic Substitute (Ames Test Negative) For Benzidine[] Suitable As Peroxidase Substrate For Use In Elisa Procedures. The Substrate Produces A Soluble End Product That Is Pale Blue In Color And Can Be Read Spectrophotometrically At 370 Or 620-650 Nm. The Tmb Reaction May Be Stopped With 2 M H2So4 (Resulting In A Yellow Color), And Read At 450 Nm. A Sensitive And Specific Reagent For The Detection Of Blood,[] Assay Of Hemoglobin,[][][] Assay Of Peroxidases.[][][]

Other Notes

Standard For Determining Aromatic Amines Released From Azo-Dyes In Commodities[1]